G418造句

By G418 screening and colonies counting, gene pool A was chosen for the second cycle transfection.

The cells were planked with lower cell density, and further screened in DMEM medium containing G418 MFD;

The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.

采用较低的细胞密度铺板，加入含最小致死量G418的DMEM培养基进行再次筛选；

经G418筛选并计集落数，选择克隆数最多的基因池进入第二轮。 如此反复进行。

用新霉素类似物(G418)筛选出阳*克隆，并用流式细胞术进一步筛选出高效表达克隆，建立稳定高效表达CHO细胞株。